Anti-p53 [Pab DO-1]

Anti p53 monoclonal antibody used to analyse and detect the most mutated gene in cancer’ p53 and its protein interactions, enabling identification of transformed cell lines.

Contributors

Inventor Institute
David Lane University of Dundee
Cat. #: 151147
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Apoptosis and autophagy;Cancer;Cell biology;Genetics
Application: ChIP ; IHC ; IF ; IP ; WB
Target: p53 (N terminus)
Reactivity: Human
Clone: Pab DO-1
Host: Mouse
Class: Monoclonal
Primary citation: Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.
Alternate name: P53, Trp53, pantropic
Product description: A monoclonal p53 antibody, the epitope recognised is in the N terminus of p53. DO-1 recognises wild-type and mutant p53. DO-1 recognises three of the p53 isoforms (p53, p53β, p53γ).
Conjugation: Unconjugated
Isotype: IgG2a
Molecular weight: 53 kDa
Immunogen: Recombinant human wild type p53 protein expressed in E.coli
Immunogen Uniprot ID: P04637
Myeloma used: Sp2/0-Ag14
Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations. Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy. Twelve isoforms of p53 have been described: p53α, p53β, p53γ, Δ40p53α, Δ40p53β, Δ40p53γ, Δ133p53α, Δ133p53β, Δ133p53γ, Δ160p53α, Δ160p53β, and Δ160p53γ. Depending on the location of the epitope, antibodies may detect different isoforms of p53. Mutations to p53 can cause conformational changes that expose different epitopes. This antibody was created by David Lane, who also discovered p53, to measure p53 in various analyses of different tissue samples. Anti-p53 DO-1 has been used frequently for staining sections, it can be used on frozen sections as well as methacarn or formol saline fixed paraffin sections. DO-1 recognises an epitope on the N terminus region of p53, between aa 20-25. DO-1 recognises both wild-type and mutant p53. DO-1 recognises three of the p53 isoforms (p53, p53β, p53γ).
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Choi et al. 2018. Proc Natl Acad Sci U S A. 115(42):10666-10671. PMID: 30266789.

Choi et al. 2018. Cell Death Dis. 9(6):633. PMID: 29795372.

Ghosh et al. 2018. Elife. 7:. PMID: 29474172.

Rizzo et al. 2016. Nucleic Acids Res. :. PMID: 27923994.

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

Ilhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Berges et al. 2016. Immunol Res. :. PMID: 26724940.

Kloet et al. 2015. Biochem J. 469(2):289-98. PMID: 25990325.

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.

Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.

Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.

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