Anti-Melphalan-modified DNA [Amp4/42]

Exclusive tool

Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali

Contributors

Inventor Institute
Michael Tilby The Institute of Cancer Research; Newcastle University
SKU: 151465
Product description: Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali
Conjugation: Unconjugated
Isotype: IgG2b
Myeloma used: Y3.AG.1.2.3
Cat. #: 151465
Provenance and Ownership: The Anti-Melphalan-modified DNA [Amp4/42] is owned by Cancer Research UK. CancerTools supplies the Anti-Melphalan-modified DNA [Amp4/42] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-Melphalan-modified DNA [Amp4/42] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer; Genetics
Application: ELISA ; IF
Target: Melphalan
Reactivity: Human ; Mouse
Clone: Amp4/42
Host: Rat
Class: Monoclonal
Target background: The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15

283(2):127-34.PMID: 12581733

Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.

Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monoFn derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.

A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.

Images

View Gallery
Adapted from McCartney H; Martin AM; Middleton PG; Tilby MJ. Antibody recognition of melphalan adducts characterized using immobilized DNA

Related Tools for Antibodies

Cat. # Tool Name
151466 Anti-Melphalan-modified DNA [MP5/73] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.