Anti-Mannan [LM21]

Contributors

Inventor Institute
Paul Knox University of Leeds
SKU: 157889
Product description: How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known in detail. It has been shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted. In secondary cell walls, mannan esterification can prevent probe recognition of epitopes/ligands, and detection of mannans in primary cell walls can be effectively blocked by the presence of pectic homogalacturonan. Masking by pectic homogalacturonan is shown to be a widespread phenomenon in parenchyma systems, and masked mannan was found to be a feature of cell wall regions at pit fields. Direct fluorescence imaging using a mannan‐specific carbohydrate‐binding module and sequential enzyme treatments with an endo‐Î?‐mannanase has confirmed the presence of cryptic epitopes and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro‐environments.
Alternate name: ?(1-4) Mannan
Conjugation: Unconjugated
Immunogen: Neoglycoprotein immunogen, prepared by coupling of mannopentaose (Man5) and digalactosylmannopentaose (Gal2Man5) oligosaccharides to bovine serum albumin (BSA) by reductive amination.
Myeloma used: IR983F
Cat. #: 157889
Unit size: 100 ug
Research Fields: Biochemistry
Target: Î?-(1→4)-manno-oligosaccharides from DP2 to DP5.
Host: Rat
Class: Monoclonal
Target background: How the diverse polysaccharides present in plant cell walls are assembled and interlinked into functional composites is not known in detail. It has been shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted. In secondary cell walls, mannan esterification can prevent probe recognition of epitopes/ligands, and detection of mannans in primary cell walls can be effectively blocked by the presence of pectic homogalacturonan. Masking by pectic homogalacturonan is shown to be a widespread phenomenon in parenchyma systems, and masked mannan was found to be a feature of cell wall regions at pit fields. Direct fluorescence imaging using a mannan‐specific carbohydrate‐binding module and sequential enzyme treatments with an endo‐Î?‐mannanase has confirmed the presence of cryptic epitopes and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro‐environments.
Format: Liquid
Shipping conditions: Dry ice
References:

Marcus et al. 2010. Plant J. 64(2):191-203. PMID: 20659281.

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