Anti-HLA-A2 [BB7.2]

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Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
SKU: 151297
Product description: Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production.
Conjugation: Unconjugated
Isotype: IgG2b
Immunogen: Papain solubilised HLA-A2,
Immunogen Uniprot ID: P04439
Myeloma used: P3/NS1/1-Ag4.1
Cat. #: 151297
Provenance and Ownership: The Anti-HLA-A2 [BB7.2] is owned by Cancer Research UK. CancerTools supplies the Anti-HLA-A2 [BB7.2] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-HLA-A2 [BB7.2] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Immunology
Application: FACS ; IHC ; IF ; Fn
Target: Human Leukocyte Antigen- A2
Reactivity: Human
Clone: BB7.2
Host: Mouse
Class: Monoclonal
Target background: Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). The polymorphic HLA-A, -B, -C glycoproteins have a molecular weight of 43,000 daltons and are expressed on the surfaces of nearly all somatic cells, except red blood cells. This antibody can be used for HLA typing. Anti-HLA-A2 BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by the A.02 allele. It recognises an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands. Anti-HLA-A2 BB7.2 binds to an epitope at the C-terminus of the alpha-2 helix within the human HLA-A2 histocompatibility antigen. This antibody has exhibited blocking activity. Anti-HLA monoclonal antibodies can help identify in vitro translation production from HLA region mRNA, alongside isolating specific mRNA via precipitation or binding of polysomes. Anti-HLA-A2 can aid study within the immune response, including the presentation of foreign antigens to the immune system.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

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Monoclonal antibodies for analysis of the HLA system.

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Adapted from Lasso et al. 2016. PLoS One. 11(3)

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