Anti-EBV Latent Membrane Protein 1 [LMPO24]

Monoclonal antibody which can be used to investigate EBV latency and EBV-associated malignant cells.

Contributors

Inventor Institute
Martin Rowe University of Birmingham
SKU: 152632
Product description: Monoclonal antibody which can be used to investigate EBV latency and EBV-associated malignant cells. Background and Research Application EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases. These include infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. This antibody is specific for latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV). LMP1 is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. This protein can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
Conjugation: Unconjugated
Isotype: IgG1 kappa
Immunogen: Purified plasma membranes from LMP1 expressing insect cells infected with a recombinant LMP1 baculovirus
Immunogen Uniprot ID: P03230
Cat. #: 152632
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Microbiology
Application: FACS ; IHC
Target: Epstein-Barr Virus, Latent Membrane Protein 1 (EBV-LMP1)
Reactivity: Virus
Clone: LMPO24
Host: Mouse
Class: Monoclonal
Target background: Monoclonal antibody which can be used to investigate EBV latency and EBV-associated malignant cells. Background and Research Application EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases. These include infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. This antibody is specific for latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV). LMP1 is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. This protein can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Niedobitek et al. 1995. Blood. 86(2):659-65. PMID: 7605996.

Heterogeneous expression of Epstein-Barr virus latent proteins in endemic Burkitt's lymphoma.

Images

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Intracellular staining of LMP1 using LMPO24 antibody. NK and T cell lines were fixed and permeabilised with an EBiosciences kit (Cat 88-8824-00) then stained with LMPO24 monoclonal antibody; followed by detection with Alexa Fluor 488 conjugated anti-mouse IgG antibody and analysis by flow cytometry. The histograms show negative control fluorescence staining (black lines) and LMPO24 staining (red lines). MEC04; SNK1; SNK6 and SNT8 are EBV positive tumour cell lines derived from patients with nasal NK/T cell lymphomas. MEC04; SNK1 and SNK6 are NK cell lines; while SNT8 is a T cell line. MEC04 lacks LMP1 expression; SNK1 expresses weak to moderate levels of LMP1; whilst SNK6 and SNT8 express high levels of LMP1.

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