E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection.
| Inventor | Institute |
|---|---|
| Julian Gannon | Cancer Research UK, London Research Institute: Clare Hall Laboratories |
| SKU: | 151303 |
|---|---|
| Product description: | Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase. |
| Alternate name: | CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2Human Entrez Gene ID: 89 Human SwissProt: P2248 Human Unigene: 58974 Human Gene Symbol: CCNA2Human Chromosome Location: 4q27 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a kappa |
| Molecular weight: | 54 kDa |
| Immunogen: | Full length bovine Cyclin A protein |
| Immunogen Uniprot ID: | P78396 |
| Cat. #: | 151303 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Cell biology;Genetics |
| Application: | FACS ; IHC ; IF ; IP ; WB |
| Target: | Cyclin A |
| Reactivity: | Bovine ; Human ; Mink ; Mouse |
| Clone: | E67.1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Target background: | The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204. Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038. Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008. Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116. Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603. Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932. Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612. Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358. |
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