Anti-cyanotoxin (MC-LR) scAb

Microcystins are cyclic heptapeptides implicated in the impairment of liver function in various animals. They have two variable amino acid residues; in the case of the cyanotoxin MC-LR the variable amino acids are Leucine (L) and Arginine (R). Microcystin-LR is the most commonly found cyanotoxin and inhibits protein phosphatase type 1 and type 2A (PP1 and PP2A) activities in the cytoplasm of liver cells, leading to hepatocytic necrosis and haemorrhaging. Chronic exposure to microcystin is a worrying phenomenon as microcystin over an extended period of time can cause cancer through the inhibition of protein phosphatases PP1 and PP2A.

Contributors

Inventor Institute
Caroline Murphy ; Richard O'Kennedy Dublin City University
SKU: 158335
Product description: Microcystins are cyclic heptapeptides implicated in the impairment of liver function in various animals. They have two variable amino acid residues; in the case of the cyanotoxin MC-LR the variable amino acids are Leucine (L) and Arginine (R). Microcystin-LR is the most commonly found cyanotoxin and inhibits protein phosphatase type 1 and type 2A (PP1 and PP2A) activities in the cytoplasm of liver cells, leading to hepatocytic necrosis and haemorrhaging. Chronic exposure to microcystin is a worrying phenomenon as microcystin over an extended period of time can cause cancer through the inhibition of protein phosphatases PP1 and PP2A.
Alternate name: MC-LR, Anti-Microcystin-LR (R66S) scAb
Conjugation: Unconjugated
Immunogen: Microcystin-LR
Immunogen Uniprot ID: TBC
Cat. #: 158335
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Biochemistry
Application: ELISA ; WB
Target: Microcystin-LR
Host: Mouse
Class: Recombinant
Target background: Microcystins are cyclic heptapeptides implicated in the impairment of liver function in various animals. They have two variable amino acid residues; in the case of the cyanotoxin MC-LR the variable amino acids are Leucine (L) and Arginine (R). Microcystin-LR is the most commonly found cyanotoxin and inhibits protein phosphatase type 1 and type 2A (PP1 and PP2A) activities in the cytoplasm of liver cells, leading to hepatocytic necrosis and haemorrhaging. Chronic exposure to microcystin is a worrying phenomenon as microcystin over an extended period of time can cause cancer through the inhibition of protein phosphatases PP1 and PP2A.
Format: Liquid
Storage conditions: Store at -20° C frozen (best practice). Avoid repeated freeze/thaw cycles. Stable at room temperature in the presence of 20% (v/v) glycerol.
Shipping conditions: Dry ice
References:

Murphy et al. 2018. J Immunol Methods. 463:127-133. PMID: 30321550.

Images

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Fig. 1. (A) The avian derived IgY molecule consists of four linked polypeptide chains; two heavy domains (containing one variable heavy region (VH); and four constant heavy regions (CH1; CH2; CH3 and CH4)) and two light domains (containing one variable light (VL) and one constant light (CL) region); linked by two disulphide bonds (shown in red) (Murphy et al.; 2016). The antigen-binding region is composed of a variable light (VL) and variable heavy (VH) domain. The antibody is segregated into a Fragment antigen binding (Fab) and Fragment crystallizable (Fc) region. (B) Single chain fragment variable (scFv) displays structurally important complementarity determining regions (CDR); and consists of VH and VL domains linked by a serine-glycine linker. (C) The single chain antibody (scAb) fragment is composed of a human kappa light chain constant domain (CL) connected to a scFv.

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