A mouse monoclonal antibody recognising human complement receptor 1/CD35, used to identify and quantify CR1-expressing cells.
| Inventor | Institute |
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| Nancy Hogg | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 151087 |
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| Product description: | Anti-CR1 [E11] is a mouse monoclonal antibody that recognises human complement receptor 1, also known as CR1 or CD35. It binds an epitope distal from the C3b binding site without blocking CR1 activity, making it useful for identifying CR1 in tissue sections and quantifying CR1 on erythrocytes and neutrophils. |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Molecular weight: | 130 kDa |
| Immunogen: | Acute monocytic leukaemia cells and normal blood monocytes. |
| Immunogen Uniprot ID: | P17927 |
| Myeloma used: | P3/NS1/1-Ag4.1 |
| Cat. #: | 151087 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer;Immunology |
| Application: | IHC ; IP |
| Target: | Complement receptor 1 (CR1, CD35) |
| Reactivity: | Human |
| Clone: | E11 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248. |
| Target background: | CR1 is found on many leucocytes and binds C3b and C4b. E11 detects CR1 on most leukemic monoblasts, granulocytes and may be used to define B cell lymphomas. E11 binds to a 222kDa antigen. This antibody is specific for a binding site in CR1 which is distal from the C3b binding site. As such CR1 activity is not blocked. CR1 is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b within the complement system. CR1 is present on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukaemia’s, follicular dendritic reticulum cells and is negative on basophils. Anti-CR1 E11 is useful for identifying CR1 on various cells in tissue sections, and for quantitating CR1 on erythrocytes and neutrophils. |
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| Format: | Liquid |
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| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Leucocyte Typing III. Edited by AJ McMichael et al. 1987 Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820. Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463. Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996. Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340. Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248. |
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