Anti-CD45RO [UCH-L1]
Exclusive ToolHighly specific monoclonal antibody which can detect CD45RO, differentiating between subpopulations of T lymphocytes.
Contributors
| Inventor | Institute |
|---|---|
| Peter Beverley | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 151173 |
|---|---|
| Product description: | Highly specific monoclonal antibody which can detect CD45RO, differentiating between subpopulations of T lymphocytes. |
| Alternate name: | Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T2; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T2 Leukocyte Common Antigen; T2 Glycoprotein; EC 3.1.3.48; GP18; CD45R; B22; LCA; LY5 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Molecular weight: | 180 kDa |
| Immunogen: | Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus. |
| Immunogen Uniprot ID: | P08575 |
| Myeloma used: | P3/NS1/1-Ag4.1 |
| Cat. #: | 151173 |
|---|---|
| Provenance and Ownership: | The Anti-CD45RO [UCH-L1] is owned by Cancer Research UK. CancerTools supplies the Anti-CD45RO [UCH-L1] under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Anti-CD45RO [UCH-L1] directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cancer; Cell biology; Immunology; Tags and cell markers |
| Application: | FACS ; IHC ; IP ; WB |
| Target: | Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO) |
| Reactivity: | Human |
| Clone: | UCH-L1 |
| Host: | Mouse |
| Class: | Monoclonal |
| Target background: | CD45RO (UCHL1) is also known as protein tyrosine phosphatase receptor type C (PTPRC) or leukocyte common antigen. It is a transmembrane pan-leukocyte protein with tyrosine phosphatase activity and is involved in the regulation of signal transduction in haematopoiesis and the threshold of T cell antigen receptor (TCR) signalling. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Elgueta et al. 2019. Front Immunol. 10:981. PMID: 31118938. Moura et al. 2017. Cell Mol Immunol. 14(9):758-769. PMID: 26996067. Wu et al. 2015. Oncotarget. 6(30):28633-45. PMID: 26416419. Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766. Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types. Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532. Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction. Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028. CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO. Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210. Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis. Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439. Fn subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
|---|
Images
View Gallery
Product Images
6a93670e-2553-4c6a-92dc-be0a7d62d145.png
Antigen cell-surface expression in CD45R0+ and CD45RBC+ HPB-ALL sub-clones.CD45R0+ (continuous line) or CD45RBC+ (dotted line) HPB-ALL cells were stained for CD45 as shown and analyzed by FACS. Adapted from Dornan et al J Biol Chem. 2002 Jan 18;277(3)
