P1H5 precipitated a single cell surface protein. The data obtained from inhibition of cell attachment studies with class II antibodies strongly suggested that attachment to collagen was mediated by this class II receptor.
| Inventor | Institute |
|---|---|
| Elizabeth A. WaynerWilliam Carter, | Fred Hutchinson Cancer Center |
| SKU: | 161652 |
|---|---|
| Product description: | MoAb is a mouse monoclonal antibody raised against HT-1080. MoAb was developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. This antibody inhibited cell adhesion to type VI and I collagens only and not to fibronectin or laminin. |
| Isotype: | IgG1 |
| Molecular weight: | 125-145 kDa |
| Immunogen: | HT1080 human fibrosarcoma cell line |
| Cat. #: | 161652 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Cell biology |
| Application: | FACS, Immunohistochemistry, Immunoprecipitation, Western Blot |
| Target: | CD44 |
| Reactivity: | Human |
| Clone: | P1H5 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Wayner et al.1987. Journal of Cell Biology.105(4):1873-84. PMID: 2822727 |
| Target background: | CD44 is a hyaluronan cell adhesion molecule (HCAM), GP90 lymphocyte homing receptor, and class III collagen receptor (CRIII). Antibody bound and inhibited cell adhesion to type VI and I collagens (and bound type III, IV, and V collagens with no inhibition) only and not to fibronectin or laminin |
|---|
| Storage conditions: | For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727 Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668 Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064 |
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