Anti-CD3 [500.A2]

Monoclonal antibody capable of activating T cells and cloned T cell lines.

Contributors

Institute
Yale University
Cat. #: 155246
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Immunology
Target: CD3
Host: Hamster
Class: Monoclonal
Product description: Monoclonal antibody capable of activating T cells and cloned T cell lines. The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. Binding of anti-CD3 (500.A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Conjugation: Unconjugated
Isotype: IgG
Immunogen: 25-kDa e chain of the T-cell receptor-associated CD3 complex
Immunogen Uniprot ID: A0A3Q0CNZ4
Target background: Monoclonal antibody capable of activating T cells and cloned T cell lines.Background and Research ApplicationThe CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. Binding of anti-CD3 (500.A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Chen et al. 2019. JCI Insight. 4(22):. PMID: 31723059.

Trittel et al. 2019. Sci Rep. 9(1):16362. PMID: 31704965.

Czarnewski et al. 2019. Nat Commun. 10(1):2892. PMID: 31253778.

Zwick et al. 2019. Front Immunol. 10:222. PMID: 30809231.

Shahbaz et al. 2018. PLoS Biol. 16(12):e2006649. PMID: 30550561.

Maj et al. 2017. Nat Immunol. 18(12):1332-1341. PMID: 29083399.

Long et al. 2016. Genes (Basel). 8(1):. PMID: 28035981.

Portoles et al. 1989. J Immunol. 142(12):4169-75. PMID: 2470817.

Related Tools for Antibodies

Cat. # Tool Name
151034 Anti-HSVUL42 [13D11] Key Info View Tool
151036 Anti-ICAM1 [15.2] Key Info View Tool
151040 Anti-CD11 & CD18 [24] Key Info View Tool
151028 Anti-Cytochrome P450 2C2, 2B1/2 [h7] Key Info View Tool
151030 Anti-Progesterone [11P14] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.