Anti-CD29 [12G10]

A monoclonal antibody recognising human CD29/integrin beta 1, used to study integrin activation, cell adhesion and cell–matrix interactions.

Contributors

Inventor Institute
Martin Humphries University of Manchester
SKU: 152482
Product description: Anti-CD29 [12G10] is a mouse monoclonal antibody that recognises human CD29, also known as integrin beta 1. It is used to study integrin activation, cell adhesion and cell–matrix interactions, supporting research into integrin-mediated signalling, immune response, tissue repair and tumour cell adhesion. Clone 12G10 recognises a ligand-induced binding site epitope on the beta 1 integrin subunit. Reported applications include ELISA, FACS, immunohistochemistry, immunofluorescence, immunoprecipitation and western blot.
Alternate name: Integrin Subunit Beta 1; Glycoprotein Iia; MSK12; GPIIA; FNRB; MDF2; Very Late Activation Protein; Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; CD29 Antigen; VLA-BETA; CD29; VLAB
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: Purified human ?1 integrin preparation from HT1080 fibrosarcoma cell extract.
Myeloma used: P3X63Ag8.653
Cat. #: 152482
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer; Cell biology; Cell signaling and signal transduction; Genetics; Immunology
Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
Target: CD29 (Integrin b1 chain)
Reactivity: Human
Clone: 12G10
Host: Mouse
Class: Monoclonal
Target background: CD29 is a member of the family of integrins, membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.
Format: Liquid
Concentration: 0.9-1.1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Chen et al. 2009. PLoS One. 4(8):e6565. PMID: 19668337.

Yamamoto et al. 2009. Endocrinology. 150(2):990-9. PMID: 18845630.

Yamamoto et al. 2007. Br J Cancer. 97(11):1538-44. PMID: 17971775.

Cavallo-Medved et al. 2005. J Cell Sci. 118(Pt 7):1493-503. PMID: 15769846.

Avizienyte et al. 2002. Nat Cell Biol. 4(8):632-8. PMID: 12134161.

Iba et al. 2000. J Cell Biol. 149(5):1143-56. PMID: 10831617.

Mould et al. 1998. Biochem J. 331 ( Pt 3):821-8. PMID: 9560310.

Mould et al. 1995. Biochem Soc Trans. 23(3):395S. PMID: 8566283.

Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.

Mould et al. 1995. FEBS Lett. 363(1-2):118-22. PMID: 7537221.

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