Anti-Cathepsin L [4C11]

It is recognized that many cancer cells secrete cathepsin L to degrade the components of extracellular matrices and basement membranes. This promotes tumor invasion and metastasis.

Contributors

Institute
A*STAR Accelerate Technologies Pte Ltd
SKU: 152642
Product description: Cathepsin L is expressed in most cells but in a higher level in cancerous cells. Normal levels of cathepsin L is involved in the regulation of epithelial cell proliferation and differentiation in the skin. It is recognized that many cancer cells secrete cathepsin L to degrade the components of extracellular matrices and basement membranes. This promotes tumor invasion and metastasis. The pro-form of cathepsin L exists as a molecular size of 50kDa. The pro-form could be degraded and secreted out as two smaller forms of cathepsin L. These smaller forms are known as the mature forms. The pro-forms and mature forms are secreted out of the healthy cells. However, there is a significant increase in levels of pro-forms that are secreted out of the cancerous cells. Monoclonal antibodies generated against pro-form of cathepsin L would be useful for diagnostic purposes.
Conjugation: Unconjugated
Isotype: IgM
Immunogen: GST-HCL fusion protein
Myeloma used: Sp2/0-Ag14
Cat. #: 152642
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Biochemistry; Cancer; Cell biology; Cell signaling and signal transduction; Neurobiology
Application: IF ; WB
Target: Cathepsin L, HCL
Reactivity: Human
Clone: 4C11
Host: Mouse
Class: Monoclonal
Target background: Cathepsin L is expressed in most cells but in a higher level in cancerous cells. Normal levels of cathepsin L is involved in the regulation of epithelial cell proliferation and differentiation in the skin. It is recognized that many cancer cells secrete cathepsin L to degrade the components of extracellular matrices and basement membranes. This promotes tumor invasion and metastasis. The pro-form of cathepsin L exists as a molecular size of 50kDa. The pro-form could be degraded and secreted out as two smaller forms of cathepsin L. These smaller forms are known as the mature forms. The pro-forms and mature forms are secreted out of the healthy cells. However, there is a significant increase in levels of pro-forms that are secreted out of the cancerous cells. Monoclonal antibodies generated against pro-form of cathepsin L would be useful for diagnostic purposes.
Format: Liquid
Concentration: 0.9-1.1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice

Images

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Immunofluorescence was performed on human brain cell line (U-138MG) using anti-Cathepsin L [4C11] (A) or a commercially sourced antibody (B)

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