Cat. #160830
Temperature sensitive Protease-V24
Cat. #: 160830
Availability: Please enquire for quantities and pricing
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: The University of Victoria
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Temperature sensitive Protease-V24
- Alternate name: V24
- Research fields: Genetics
- Description: This novel temperature sensitive protease, a variant of subtilisin Carlsberg (SubC) protease, is expressed in Bacillus subtilis. Variant 24 (V24) have been shown to be capable of degrading a broad range of proteins in their native or denatured state. V24 has an inactivation profile that allows protease digestion at 40°C and inactivation at 50°C. All activity is destroyed after 10 minutes of incubation at 50°C (See Figure above). This variant has proven valuable in working with fragile specimen in which minimal heat treatment is desired. Due to the unique properties of V24 it has also been shown to improve molecular biology research, automated processes like Next-Gen Sequencing and disease diagnostics workflows. No column purification or treatment at extreme temperatures are required to completely deactivate this variant. This protease remains active in the presence of detergent such as SDS and Triton X-100.
- Additional notes: Canadian Patent Application No. 3028612 US Patent No. 10894954
Handling
- Storage conditions: Activity remains stable over several months when the protease is stored at 4° C or lower. When stored at -80° C the protease remains stable for up to two years.
Related Tools
- Related tools: Temperature sensitive Protease-V25
References
- Thompson et al. 2020. FEMS Microbiol Lett. 367(19):. PMID: 33016320.
