CRUK1061 T1-R5 TRACERx lung cancer PDX

CRUK1061 T1-R5-PDX: Sample from primary NSCLC tumour collected from a White-British male, with a primary diagnosis of lung squamous cell carcinoma (stage 3a), ex-smoker

Contributors

Inventor Institute
Robert Hynds, David Pearce CRUK Lung Cancer Centre of Excellence at University College London
Cat. #: 162279
Cancer types: Lung cancer
Cancer Subtype: NSCLC: Lung Squamous Cell Carcinoma
Gender: Male
Age, y.o.: 75
Cancer Stage or Grade: 3a
Biopsy Site: Primary lung resection
Patient ethnicity: White-British
Treatment History: Naive
Primary citation: Hynds R.E. et al., Nature Communications 15, 4653 (2024). PMID: 38821942
Alternate name: CRUK1061_PDX_T1-R5
Product description: CRUK1061 T1-R5-PDX: Sample from primary NSCLC tumour collected from a White-British male, with a primary diagnosis of lung squamous cell carcinoma (stage 3a), ex-smoker
Initial handling information: Primary NSCLC tumour material was minced and injected subcutaneously in the flank of male NSG mice in growth factor reduced matrigel.
Additional notes: Additional information on PDX model establishment and characterisation can be found in the Product Information Sheet.
Mice Passaged: Yes
Engraftment Site: Subcutaneous (flank)
Sample Type: Minced tumour
Host Strain: NOD scid gamma (NSG) mice
Production details: Primary NSCLC tumour material was minced and injected subcutaneously in the flank of male NSG mice in growth factor reduced matrigel. To prepare frozen samples for injection, the sample cryovial was warmed in a 37°C water bath until just thawed. The whole sample was then transferred into a sterile 1.5 ml centrifuge tube and topped up with transport medium. The sample was centrifuged at 300 x g for 5 minutes and the supernatant was removed. The sample was then washed with transport medium. If required, any large pieces of tissue were collected and finely minced with a scalpel to ensure they could pass through the needle. The sample was then centrifuged again and gently resuspended in ice-cold Matrigel, and kept on ice before subcutaneous injection. When tumours reached 1.5 cm3 in volume (calculated as 0.5 x length x width2), tumours were aseptically collected and reimplanted into new mice or cryopreserved and banked.
References:

Hynds R.E. et al., Nature Communications 15, 4653 (2024). PMID: 38821942

Jamal-Hanjani M. et al.,PLoS Biol.12, e1001906 (2014). PMID: 25003564

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