ZR-75-1 [IX-10] cell line

Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Retroviral insertion mutagenesis was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method the main genes conferring estrogen independence […]

Contributors

Inventor Institute
Lambert Dorssers Erasmus University Medical Center (Erasmus MC)
Cat. #: 154549
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Gynaecologic cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Cancer Model
Cancer Types In Detail: Mammary ductal carcinoma
Product description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Retroviral insertion mutagenesis was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method the main genes conferring estrogen independence in human breast cancer cells where identified. Genes located in the immediate proximity of the retroviral integration site were characterised. Out of 15 candidate breast cancer antigen resistance (BCAR) genes, seven (AKT1, AKT2, BCAR1, BCAR3, EGFR2, GRB7 and TRERF1/BCAR2) were shown to directly underline estrogen independence. Cell line with unique integration events in the following genes, of which one is the most likely cause for estrogen independence: ZC3HDC5 and PSME4This cell line is part of a panel of 71 cell lines (Cat No 154549-154619) plus the parental (Cat No 154547).These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity.
Production details: ZR-75-1 cells were infected with amphotropic, defective murine retrovirus and plated in medium containing 1uM of 4-hydroxy-tamoxifen. Within 5 weeks after the start of selection proliferating colonies were individually picked and expanded to stable cell lines
Parental cell line: ZR-75-1
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS)
Mycoplasma free: Yes
Biosafety level: 1
References:

van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.

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