UM-UC-7 (Human transitional cell carcinoma of the bladder) cell line

UM-UC-7 is a human transitional cell carcinoma cell line derived from a male bladder cancer patient. The UM-UC panel of urothelial carcinoma is a diverse range of 11 patient derived cell lines developed by the university of Michigan that have been well-categorised in the literature.

Contributors

Inventor Institute
H. Grossman ; Anita Sabichi University of Michigan
Cat. #: 160438
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Bladder cancer
Research Fields: Cancer;Cancer
Organism: Human
Tissue: Bladder
Gender: Male
Model: Tumourigenic cell line
Growth properties: Adherent
Cancer Types In Detail: Human bladder transitional cell carcinoma;Human bladder transitional cell carcinoma
Alternate name: UMUC7; University of Michigan-Urothelial Carcinoma-7
Product description: UM-UC-7 is a human transitional cell carcinoma cell line derived from a male bladder cancer patient. The UM-UC panel of urothelial carcinoma is a diverse range of 11 patient derived cell lines developed by the university of Michigan that have been well-categorised in the literature. These cells were assessed for their susceptibility to adenoviral mediated gene delivery, tumour growth in nude mice and differences in genetic alterations.
Gender: Male
CRISPR: Yes
Conditional: Yes
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells were grown in EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm2; using 0.05% trypsin/EDTA; 5% CO2, 37°C. Doubling time approximately 19 hours.
Mycoplasma free: Yes
References:

Grossman et al. 1986. J Urol. 136(5):953-9. PMID: 3761468

Park et al. 2008. J Natl Cancer Inst. 100(19):1401-11. PMID: 18812553

Guo et al. 2013. J Pathol. 230(1):17-27. PMID: 23401075

Allory et al. 2014. Eur Urol. 65(2):360-6. PMID: 24018021

Earl et al. 2015. BMC Genomics. 16:403. PMID: 25997541

Zuiverloon et al. 2018. Bladder Cancer. 4(2):169-83. PMID: 29732388

Images

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UM-UC-7 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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