UM-RC-3 (Human renal carcinoma) cell line

UM-RC-3 are a renal carcinoma cell line, established in long term culture, from surgically obtained renal carcinoma specimens of patients who underwent nephrectomy. This patient had metastatic disease at the time of nephrectomy. Initial work suggests these cells grow well in primary culture but life expectancy is limited to less than 10 passages.

Contributors

Inventor Institute
H. Grossman University of Michigan
Cat. #: 160447
Unit size: 1×10^6 cells / vial
Cancer types: Kidney cancer
Research Fields: Cancer
Organism: Human
Tissue: Kidney
Model: Cancer Model
Growth properties: Adherent
Cancer Types In Detail: Human renal carcinoma;Kidney;Renal
Alternate name: UMRC-3; UMRC3; University of Michigan-Renal Carcinoma-3
Product description: UM-RC-3 are a renal carcinoma cell line, established in long term culture, from surgically obtained renal carcinoma specimens of patients who underwent nephrectomy. This patient had metastatic disease at the time of nephrectomy. Initial work suggests these cells grow well in primary culture but life expectancy is limited to less than 10 passages. These cells were evaluated for their ability to form tumors in nude mice and were confirmed to produced clear cell tumors. UM-RC-3 cells demonstrated no autologous reactivity in Protein A assays in low passage number cells but did in higher passage numbers. These cells are adherent and display a loss of contact inhibition. Doubling time is approximately 29 hours.
Conditional: No
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells were grown in Eagle's Minimum Essential Medium (EMEM) containing 1% nonessential amino acids, 2mM glutamine, 100U/ml penicillin, 100ug/ml streptomycin and 10% Fetal Bovine Serum (FBS).
References:

Wahl et al. 1991. 67(6):1544-1550. PMID: 2001543.

Cancer. Grossman et al. 1985. J Surg Oncol. 28(3):237-244. PMID: 4038766.

Images

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UM-RC-3 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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