SVCT-MI2 Cell Line

SVCT-MI2 was established in 1987 from epithelial cells in breast milk secretions.

Contributors

Inventor Institute
Joyce Taylor-Papadimitriou ; Sidney Chang Cancer Research UK, Lincoln's Inn Fields Institute
Cat. #: 152596
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell biology;Drug development;Genetics
Organism: Human
Tissue: Breast
Gender: Female
Growth properties: Adherent
Cancer Types In Detail: Breast cancer
Primary citation: Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.
Product description: SVCT-MI2 has been established from human breast epithelial cells being transfected with simian virus 40 (SV40) – a known oncogenic DNA virus in addition to a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. This transformed epithelial cell line shows tumourigenicity in nude mice (post-selection of G418-resistant colonies). SVCT-MI2 enables molecular analysis of SV40 in human breast cancers.
Gender: Female
Conditional: No
Cellosaurus ID: CVCL_2719
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10ug / ml insulin + 5ug / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA. Note: cells may pile up when confluent.
Temperature: 37° C
Atmosphere: 5% CO2
References:

Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.

Images

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SVCT-MI2 Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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