#152596

SVCT-MI2 Cell Line

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Cat. #152596

SVCT-MI2 Cell Line

Cat. #: 152596

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Breast

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Joyce Taylor-Papadimitriou ; Sidney Chang

Institute: Cancer Research UK, Lincoln's Inn Fields Institute

Primary Citation: Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: SVCT-MI2 Cell Line
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer;Cell biology;Drug development;Genetics
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Adherent
  • Conditional: No
  • Description: SVCT-MI2 has been established from human breast epithelial cells being transfected with simian virus 40 (SV40) - a known oncogenic DNA virus in addition to a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. This transformed epithelial cell line shows tumourigenicity in nude mice (post-selection of G418-resistant colonies). SVCT-MI2 enables molecular analysis of SV40 in human breast cancers.
  • Application: Molecular analysis of SV40 in breast cancer
  • Cellosaurus id: CVCL_2719

Target Details

  • Target: Human oncogene EJ Harvey-ras

Applications

  • Application: Molecular analysis of SV40 in breast cancer

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10ug / ml insulin + 5ug / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA. Note: cells may pile up when confluent.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: SVCT Cell Line

References

  • Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.