SK-N-BE(2)-C Cell Line

SK-N-BE(2)-C is a clonal subline of the SK-N-BE(2) neuroblastoma cell line. Like the parental cell line, these cells display MYCN amplification. Treatment with trans-retinoic acid differentiates these cells into a distinct neuronal phenotype. These cells display high levels of tyrosine hydroxylase activity and dopamine-b-hydroxylase activity.

Contributors

Inventor Institute
June L. Biedler, Barbara A. Spengler Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161922
Cancer types: Neurological cancer
Organism: Human
Tissue: Brain
Gender: Male
Model: Tumourigenic
Morphology: Neuroblast
Growth properties: Adherent
Cancer Types In Detail: Neuroblastoma
Primary citation: Ciccarone et al. 1989. Cancer Research. 49: 219-225. PMID: 2535691.
Product description: SK-N-BE(2)-C is a clonal subline of the SK-N-BE(2) neuroblastoma cell line. Like the parental cell line, these cells display MYCN amplification. Treatment with trans-retinoic acid differentiates these cells into a distinct neuronal phenotype. These cells display high levels of tyrosine hydroxylase activity and dopamine-b-hydroxylase activity.
Gender: Male
Additional notes: BE(2)-C cells have a reported saturation density of greater than 5 X 105 cells/cm2. The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites). Unlike the parent line, they generally do not detach and float.
Parental cell line: SK-N-BE(2)
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: 1:1 mixture of EMEM:F12 Medium, then supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Qiao et al. 2012. Biochemical and Biophysical Research Communications. 424: 421-426. PMID: 22766505.

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