SK-MEL-28 Cell Line

SK-MEL-28 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras. It is also able to form tumours in nude mice.

Contributors

Inventor Institute
Lloyd J. Old, Toshitada Takahashi Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161917
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Male
Model: Tumourigenic
Morphology: Polygonal
Growth properties: Adherent
Primary citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Product description: SK-MEL-28 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras. It is also able to form tumours in nude mice.
Gender: Male
Model description: Tumourigenic in nude mice; forms malignant melanoma (large round cell type)
Products or characteristics of interest: Karyotype: modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell. Isoenzymes: AK-1, 1-2; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Subculture routine: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.

Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.

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