SK-MEL-28 Cell Line

Contributors

Inventor Institute
Lloyd J. Old, Toshitada Takahashi Memorial Sloan-Kettering Cancer Center (MSK)
SKU: 161917
Product description: SK-MEL-28 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras. It is also able to form tumours in nude mice.
Gender: Male
Model description: Tumourigenic in nude mice; forms malignant melanoma (large round cell type)
Products or characteristics of interest: Karyotype: modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell. Isoenzymes: AK-1, 1-2; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1
Cat. #: 161917
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Male
Model: Tumourigenic
Morphology: Polygonal
Growth properties: Adherent
Primary citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Subculture routine: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.

Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.

Related Tools for Cell lines

Cat. # Tool Name
151447 CMT 64 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.