SK-MEL-2 Cell Line

Contributors

Inventor Institute
Lloyd J. Old, Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
SKU: 161915
Product description: SK-MEL-2 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and mutant N-Ras (Q61R).
Gender: Male
Model description: Tumourigenic in nude mice; forms malignant melanoma
Products or characteristics of interest: Karyotype: (P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1
Cat. #: 161915
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Male
Model: Tumourigenic
Morphology: Polygonal
Growth properties: Adherent
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359

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