RT112/84 Cell Line
The RT112/84 cell line is derived from human bladder carcinoma.
Contributors
| Inventor | Institute |
|---|---|
| Chris Marshall | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 153250 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Genitourinary cancer |
| Research Fields: | Cancer;Drug development |
| Organism: | Human |
| Tissue: | Bladder |
| Model: | Tumour line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Human bladder carcinoma epithelial |
| Primary citation: | Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752. |
| Alternate name: | RT112-84; RT-112/84; RT11284 |
|---|---|
| Product description: | Human bladder transitional cell carcinoma cell line characterised by high E-cadherin expression and strong epithelial adhesion properties. Demonstrates limited invasive capacity under standard conditions, with invasion significantly enhanced by E-Cadherin disruption (via antibodies). A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies. |
| Conditional: | Yes |
| Additional notes: | STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17 |
| Cellosaurus ID: | CVCL_2714 |
| disease: | Cancer |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture routine: | Split sub-confluent cultures (70-80%) seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Subculture weekly – cells are difficult to trypsinise, and should be kept sub-confluent. |
| References: |
Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752 Davies et al. Anticancer Res. 1999 Jan-Feb 19(1A):547-52. PMID: 10226596 Wang et al. Cancer Genet Cytogenet. 1995 Jul 15:82(2):170-4. PMID: 7664249 Fujiyama et al. Br J Cancer. 2001 Feb:84(4):558-64. PMID: 11207054 Rothenberg et al. Cancer Res. 2010 Mar 15:70(6):2158-64. Epub 2010 Mar 9. PMID: 20215515 Awsare et al. Oncol Rep. 2011 Jun:25(6):1503-9. Epub 2011 Apr 4. PMID: 21468549 Barretina et al. Nature. 2012 Mar 28:483(7391):603-7. Nature. 2012 Dec 13:492(7428):290. PMID: 22460905 Weitzet al. Front Oncol. 2017 Aug 7:7:161. PMID: 28824873 Zuiverloon et al. Bladder Cancer. 2018 Apr 26:4(2):169-183. PMID: 29732388 Ghandi et al. Nature. 2019 May:569(7757):503:508. Epub 2019 May 8. PMID: 3106870 |
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