#153250

RT112/84 Cell Line

Cat. #153250

RT112/84 Cell Line

Cat. #: 153250

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Bladder

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Chris Marshall

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: RT112/84 Cell Line
  • Cancer: Genitourinary cancer
  • Cancers detailed: Human bladder carcinoma epithelial
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Bladder
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: A slow growing tumour which does not form colonies in soft agar. Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies.
  • Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17
  • Cellosaurus id: CVCL_2714

Applications

  • Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17

Handling

  • Format: Frozen
  • Growth medium: Subculture Routine: Split sub-confluent cultures (70-80%) seeding at 2-4x10,000 cells/cm?‹Â› using 0.25% trypsin/EDTA; 5% CO2; 37?‚°C. Subculture weekly - cells are difficult to trypsinise, and should be kept sub-confluent.Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752.