RPE1 PBRM1 KO2

RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16)

Contributors

Inventor Institute
Jessica Downs, Karen Lane The Institute of Cancer Research
Cat. #: 161857
Organism: Human
Tissue: retinal epithelium
Gender: Female
Model: Genetically modified ; immortalised non-cancer cells
Morphology: Epithelial
Growth properties: Adherent
Product description: RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
Gender: Female
CRISPR: Yes
Conditional: No
Cellosaurus ID: CVCL_4388
Parental cell line: RPE1-hTERT
Model description: PBRM1 knockout
Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM /Ham F12 nutrient mixture + 10S + 1% Penicillin/Streptomycin
Temperature: 37C
Atmosphere: 5% CO2 in air
Biosafety level: 1
References:

Unpublished

Related Tools for Cell lines

Cat. # Tool Name
151463 Omega-E Packaging Cell Line Key Info View Tool
151503 1-7 CE1 Cell Line Key Info View Tool
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool
151630 PDVC57B Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.