#161857

RPE1 PBRM1 KO2

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Cat. #161857

RPE1 PBRM1 KO2

Cat. #: 161857

Availability: 8-10 weeks

Organism: Human

Tissue: retinal epithelium

Model: Genetically modified ; immortalised non-cancer cells

£575.00

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Contributor

Inventor: Jessica Downs, Karen Lane

Institute: The Institute of Cancer Research

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: RPE1 PBRM1 KO2
  • Parental cell: RPE1-hTERT
  • Clone: 2 (also called cloneB16)
  • Organism: Human
  • Gender: Female
  • Tissue: retinal epithelium
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Genetically modified ; immortalised non-cancer cells
  • Model description: PBRM1 knockout
  • Crispr: Yes
  • Conditional: No
  • Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
  • Description: RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
  • Application: Chromatin remodelling biology studies
  • Biosafety level: 1
  • Cellosaurus id: CVCL_4388

Applications

  • Application: Chromatin remodelling biology studies

Handling

  • Format: Frozen
  • Growth medium: DMEM /Ham F12 nutrient mixture + 10S + 1% Penicillin/Streptomycin
  • Temperature: 37C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 1% Penicillin/Streptomycin
  • Str profiling: See Cellosaurus profile of parental cell line

References

  • Unpublished

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