Cat. #157703
Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4
Cat. #: 157703
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Eye
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Allen Liu
Institute: University of Michigan
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4
- Research fields: Cell signaling and signal transduction
- Parental cell: Retinal Pigmented Epithelial cell line
- Organism: Human
- Tissue: Eye
- Description: Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics.
- Production details: Stable RPE cell lines expressing HA-FLAG-CXCR4 or HA-FLAG-CXCR4-MYC receptors were generated using lentiviral transduction produced from the pLVX vector
Target Details
- Target: CXCR4
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
