Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4

Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics.

Contributors

Inventor Institute
Allen Liu University of Michigan
SKU: 157703
Product description: Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics.
Production details: Stable RPE cell lines expressing HA-FLAG-CXCR4 or HA-FLAG-CXCR4-MYC receptors were generated using lentiviral transduction produced from the pLVX vector
Parental cell line: Retinal Pigmented Epithelial cell line
Cat. #: 157703
Unit size: 1×10^6 cells / vial
Research Fields: Cell signaling and signal transduction
Organism: Human
Tissue: Eye
Format: Frozen
Shipping conditions: Dry ice

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