RCC-FG2 + PBRM1 Clone 13

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: RCC-FG2 + PBRM1 Clone 13
• Research fields: Cancer
• Parental cell: RCC-FG2
• Clone: 13
• Organism: Human
• Gender: Male
• Tissue: kidney
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Genetically modified
• Model description: Conditional re-expression of wild type PBRM1 gene
• Crispr: Yes
• Conditional: Yes
• Conditional description: Conditional expression of wild type PBRM1 gene following deoxycycline treatment
• Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
• Description: RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
• Application: Chromatin remodelling biology studies
• Biosafety level: 1
• Cellosaurus id: CVCL_5873

Applications

• Application: Chromatin remodelling biology studies

Handling

• Format: Frozen
• Growth medium: RPMI-1640 + 10S + 1% Penicillin/Streptomycin
• Temperature: 37C
• Atmosphere: 5% CO2 in air
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Cultured in antibiotics: 1% Penicillin/Streptomycin
• Str profiling: See Cellosaurus profile of parental cell line

References

• Unpublished