PC346C 21 [RD D] CRPC Cell line

CRPC PC346C cell lines (27 sublines made resistant to common antiandrogens) – RD162 D

Contributors

Inventor Institute
Wytske van Weerden Erasmus University Medical Centre
Cat. #: 161831
Cancer types: Genitourinary cancer
Research Fields: Cancer
Organism: Human
Tissue: Transurethral resection of the prostate (TURP)
Gender: Male
Primary citation: Moll et al. 2022. Frontiers in oncology. 12:877613. PMID: 35769712
Product description: CRPC PC346C cell lines (27 sublines made resistant to common antiandrogens) – RD162 D
Gender: Male
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Prostate growth medium (PGM): DMEM-F12, 1X insulin-transferrin-selenium, 100 ng/mL fibronectin, 20 μg/mL fetuin, 0.01% BSA, 10 ng/mL epidermal growth factor, 50 ng/mL cholera toxin, 100µM phosphorylethanolamine, 0.6 ng/mL triiodothyronine, 0.1nM R1881, 10μM Y-27632 dihydrochloride, 0.5 µg/mL hydrocortisone and penicillin/streptomycin antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). Supplemented with 2% DCC (dextran-treated charcoal stripped FCS to remove all steroids), and 1uM RD162/ENZA.
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: PGM+20C+7.5% DMSO
Biosafety level: 2

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