OVCAR-8/EGFP_ LC3 – mCherry_ Smac
Ovarian cancer cell line OVCAR-8 stably expressing both Smac ?‚– mCherry and EGFP-LC3 for use as a dual sensor for apoptosis and autophagy.
Contributors
| Inventor | Institute |
|---|---|
| T.R. Santhosh Kumar | Rajiv Gandhi Centre for Biotechnology |
| Cat. #: | 161154 |
|---|---|
| Cancer types: | Gynaecologic cancer |
| Research Fields: | Cancer;Disease (not cancer);Metabolism |
| Organism: | Human |
| Tissue: | Ovary |
| Gender: | Female |
| Model: | Transgenic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Ovarian carcinoma |
| Primary citation: | Lupitha SS, et.al. PMID: 32109534. |
| Product description: | Ovarian cancer cell line OVCAR-8 stably expressing both Smac ? mCherry and EGFP-LC3 for use as a dual sensor for apoptosis and autophagy. |
|---|---|
| Gender: | Female |
| CRISPR: | No |
| Conditional: | No |
| Parental cell line: | OVCAR-8 |
| disease: | Cancer |
| Initial handling information: | Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2. |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated). |
| Subculture routine: | Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm? . |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Storage medium: | Growth medium + 10% DMSO |
| References: |
Lupitha SS, et.al. PMID: 32109534. |
|---|
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