Mouse fibrosarcoma Luciferase2 mStrawberry VEGF164 Cell Line

Exclusive Tool

Mouse fibrosarcoma cell lines that are capable of expressing all vascular endothelial growth factor (VEGF) isoforms (control) or only single isoforms of VEGF (VEGF120, VEGF164, or VEGF188) were developed under endogenous VEGF promoter control.

Contributors

Inventor Institute
Gillian Tozer University of Sheffield
SKU: 152854
Product description: Mouse fibrosarcoma cell lines that are capable of expressing all vascular endothelial growth factor (VEGF) isoforms (control) or only single isoforms of VEGF (VEGF120, VEGF164, or VEGF188) were developed under endogenous VEGF promoter control. The original rationale for the development of these cell lines relates to the fact that tubulin-binding vascular-disrupting agents (VDA) are currently in clinical trials for cancer therapy but the factors that influence tumour susceptibility to these agents are poorly understood. Researchers evaluated the consequences of modifying tumour vascular morphology and function on vascular and therapeutic response to combretastatin-A4 3-O-phosphate (CA-4-P), which was chosen as a model VDA. The cell lines themselves could be potentially valuable for the commercial/pharmaceutical industry.
Alternate name: VEGF-A, VEGF12, VEGF164, VEGF188
Conditional: No
Production details: Using Cre/Lox technology, mice expressing all or only single isoforms of VEGF, known as Vegfa120/120, Vegfa164/164, and Vegfa188/188 mice were developed. Primary fibroblasts were isolated from mouse embryos that were produced by heterozygous breeding pairs of mice expressing single or all isoforms of vascular endothelial growth factor-A (VEGF-A) on Swiss background. Fibroblasts were immortalized and oncogenically transformed by retroviral transduction with SV4…
Cellosaurus ID: CVCL_HG21
Parental cell line: MEF
Disease: Cancer
Cat. #: 152854
Provenance and Ownership: The Mouse fibrosarcoma Luciferase2 mStrawberry VEGF164 Cell Line is owned by Cancer Research UK. CancerTools supplies the Mouse fibrosarcoma Luciferase2 mStrawberry VEGF164 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Mouse fibrosarcoma Luciferase2 mStrawberry VEGF164 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer; Cell signaling and signal transduction; Developmental biology; Drug development; Metabolism
Organism: Mouse
Tissue: Embryo
Model: Immortalised Line
Growth properties: Adherent
Format: Frozen
Shipping conditions: Dry ice
Growth medium: High glucose DMEM (Invitrogen) medium, L-glutamine, FCS, G-418 and puromycin. antibiotics G-418 and puromycin
References:

Differential Expression of VEGFA Isoforms Regulates Metastasis and Response to Anti-VEGFA Therapy in Sarcoma. English et al. 2017. Cancer Res. 77(10):2633-2646. PMID: 28377452.

Differential Expression of VEGFA Isoforms Regulates Metastasis and Response to Anti-VEGFA Therapy in Sarcoma.

Related Tools for Cell lines

Cat. # Tool Name
152852 Mouse fibrosarcoma Luciferase2 mStrawberry VEGFWT (Control) Cell Line Key Info View Tool
152853 Mouse fibrosarcoma Luciferase2 mStrawberry VEGF120 Cell Line Key Info View Tool
152855 Mouse fibrosarcoma Luciferase2 mStrawberry VEGF188 Cell Line Key Info View Tool
153240 Mouse fibrosarcoma VEGF164 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.