Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO2. Medium is replaced twice per week.
Primary citation:
Jungwirth et al. 2021. Cancer Lett. 506:1-10. PMID: 33652084.
Product description:
NCH93 is a patient-derived meningioma cell line established from the first relapse of a WHO grade III meningioma. Tumour tissue was obtained during surgical resection from a 64 year old male patient.
Production details:
Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium:
DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine:
Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature:
37°C
References:
Yu et al. 2021. Cancers. 13(23):5898. PMID: 34885009.
Jungwirth et al. 2021. Cancers. 28:506:1-10, PMID: 33652084.
Jungwirth et al. 2019. Cancers. 11(4):545. PMID: 30991738.
Jungwirth et al. 2021. Cancer Lett. 506:1-10. PMID: 33652084.
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