Cat. #151576
MEF RAF1 KO Cell Line
Cat. #: 151576
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Mouse
Tissue: Embryo
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MEF RAF1 KO Cell Line
- Alternate name: Raf-1-/- MEF; MEF RAF1 KO
- Research fields: Cancer;Cell signaling and signal transduction;Drug development;Metabolism
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Model: Knock-Out
- Conditional: Yes
- Description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
- Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4ÄÂ?°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
- Biosafety level: 1
- Cellosaurus id: CVCL_6D89
Target Details
- Target: RAF1
Applications
- Application notes: See the Raf-1 knockout mouse in related products.
Handling
- Format: Frozen
- Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
- Mycoplasma free: Yes
Related Tools
- Related tools: Raf-1 KO Mouse
References
- Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
- MEK kinase activity is not necessary for Raf-1 function.
