#151846

MEF OGG1 KO Cell Line

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Cat. #151846

MEF OGG1 KO Cell Line

Cat. #: 151846

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Tomas Lindahl

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF OGG1 KO Cell Line
  • Research fields: Cancer;Drug development;Genetics
  • Organism: Mouse
  • Tissue: Embryo
  • Model: Knock-Out
  • Description: The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome.
  • Cellosaurus id: CVCL_6E74

Target Details

  • Target: OGG1

Applications

  • Application notes: Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Hams F-12 (3:1) with 10% FBS and genotyped by Southern hybridization, and permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture.

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F-12 (3:1) with 10% FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: OGG1 KO Mouse

References

  • Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.
  • Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.