#157986

MCF10A-HER2

Cat. #157986

MCF10A-HER2

Cat. #: 157986

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Tissue: Breast

Disease: Cancer

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Richard Grose ; Gabriella Ficz ; Ateequllah Hayat

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MCF10A-HER2
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer
  • Tool sub type: Continuous
  • Parental cell: MCF10A
  • Tissue: Breast
  • Disease: Cancer
  • Model: Immortalised Line
  • Conditional: Yes
  • Conditional description: Conditional HER2 expression under tetracyline response element (TRE) regulated by the addition of doxycyline in breast epithelial cell line (MCF10A)
  • Description: Expressed at low levels in normal breast tissues but overexpressed in a subset of breast and ovarian cancers. The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1 promoter whereas the HER2 is induced by a strong TRE promoter.
  • Production details: Inducible HER2 construct was stably transduced in MCF10A cell line using a third generation lentiviral transduction system.
  • Recommended controls: Any other HER2 cell line such as BT474 may be used as a positive control

Target Details

  • Target: Human Epidermal Receptor 2 (HER2/EBB2/neu), overexpressed in 25% breast cancers, treatments include Trastuzumab and Lapatinib. This plasmid also contains GFP for selection and tracking purposes.

Applications

  • Application notes: The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1a promoter whereas the HER2 is induced by a strong TRE promoter.

Handling

  • Format: Frozen
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice