MCF10A-HER2

The MCF10A-HER2 cell line expresses HER2 which is expressed at low levels in normal breast tissues but overexpressed in a subset of breast and ovarian cancers.

Contributors

Inventor Institute
Richard Grose ; Gabriella Ficz ; Ateequllah Hayat Queen Mary University of London
Cat. #: 157986
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Immortalised Line
Cancer Types In Detail: Breast cancer
Product description: This cell line overexpresses HER2 which is expressed at low levels in normal breast tissues but is overexpressed in a subset of breast and ovarian cancers. The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1 promoter whereas the HER2 is induced by a strong TRE promoter.
Gender: Female
Conditional: Yes
Conditional description: Conditional HER2 expression under tetracyline response element (TRE) regulated by the addition of doxycyline in breast epithelial cell line (MCF10A)
Production details: Inducible HER2 construct was stably transduced in MCF10A cell line using a third generation lentiviral transduction system.
Parental cell line: MCF10A
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Recommended controls: Any other HER2 cell line such as BT474 may be used as a positive control

Citations And Reviews

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