MCF10A-ERRB2m-9 cell line

Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, ERBB2 (erb-B2 Receptor Tyrosine Kinase 2).

Contributors

Inventor Institute
Medical-Industrial Translational Research Center Fukushima Medical University
Cat. #: 161198
Tool sub type: Continuous
Research Fields: Cancer;Cell biology
Organism: Human
Tissue: Mammarygland epithelium
Model: Mutant
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Non-tumorigenic
Primary citation: Irie et al. Mol Cancer Ther. 2019 Apr, 18(4):733-742. PMID: 30787176
Alternate name: 06M15004
Product description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, ERBB2 (erb-B2 Receptor Tyrosine Kinase 2). ERBB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.
Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
Model description: Type-10; Type mutation: V777L; CDS mutation: c.2329G>T; AA mutation: p.V777L; COSMIC Mutation ID: COSM14062
Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
Temperature: 37° C
Atmosphere: Humidified incubator with 5% CO2
Storage medium: CELLBANKER 2 (Zenogen pharma)
Mycoplasma free: Yes
Biosafety level: 1
References:

Irie et al. Mol Cancer Ther. 2019 Apr, 18(4):733-742. PMID: 30787176

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