MCF10A-ERRB2m-5 cell line

Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, ERBB2 (erb-B2 Receptor Tyrosine Kinase 2).

Contributors

Inventor Institute
Medical-Industrial Translational Research Center Fukushima Medical University
SKU: 161194
Product description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, ERBB2 (erb-B2 Receptor Tyrosine Kinase 2). ERBB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.
Alternate name: 06M15009
Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
Model description: Type-06; Type mutation: A775_G776insYVMA; CDS mutation: c.2324_2325ins12(ATACGTGATGGC); AA mutation: p.A775_G776insYVMA; COSMIC Mutation ID: COSM20959
Cat. #: 161194
Tool sub type: Continuous
Research Fields: Cancer; Cell biology
Organism: Human
Tissue: Mammarygland epithelium
Model: Mutant
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Non-tumorigenic
Primary citation: Irie et al. Mol Cancer Ther. 2019 Apr, 18(4):733-742. PMID: 30787176
Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM/Ham’s F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
Temperature: 37° C
Atmosphere: Humidified incubator with 5% CO2
Storage medium: CELLBANKER 2 (Zenogen pharma)
Mycoplasma free: Yes
Biosafety level: 1
References:

Irie et al. Mol Cancer Ther. 2019 Apr, 18(4):733-742. PMID: 30787176

Images

View Gallery

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.