MCF10A-EGFR1m-10 cell line
Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. EGFR (epidermal growth factor receptor).
Contributors
| Inventor | Institute |
|---|---|
| Medical-Industrial Translational Research Center | Fukushima Medical University |
| SKU: | 161177 |
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| Product description: | Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. EGFR (epidermal growth factor receptor). EGFR is a trans-membrane type receptor tyrosine kinase and signaling caused by EGFR is thought to play a crucial role in maintaining homeostasis of normal tissues by being involved in the regulation of cells such as proliferation and differentiation. Mutation of EGFR gene is found in lung cancer and it is known that its constant activation causes cell proliferation and tumorigenesis. Deletion of the Exon19 region, mutation of L858R and T790M are known as somatic cell mutations often found in cancer. |
| Alternate name: | 06M01027 |
| Cellosaurus ID: | CVCL_C7QB |
| Parental cell line: | MCF10A, a non-tumorigenic cell line from human mammarygland epithelium |
| Model description: | Type-22; Type mutation: H773_V774insPH; CDS mutation: c.2319_2320insCCCCAC; AA mutation: p.H773_V774insPH; COSMIC Mutation ID: COSM12380 |
| Cat. #: | 161177 |
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| Tool sub type: | Continuous |
| Organism: | Human |
| Tissue: | Mammarygland epithelium |
| Model: | Mutant |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Non-tumorigenic |
| Primary citation: | Hoshi et al. Oncol Rep. 2017 Jan, 37(1):66-76. PMID: 27840983 |
| Initial handling information: | Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days. |
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| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM/Ham’s F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin |
| Temperature: | 37° C |
| Atmosphere: | Humidified incubator with 5%Â CO2 |
| Storage medium: | CELLBANKER 2 (Zenogen pharma) |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Hoshi et al. Oncol Rep. 2017 Jan, 37(1):66-76. PMID: 27840983 |
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