MCF-7 scrambled RNA Control cell lineÂÂ
| Inventor | Institute |
|---|---|
| Priya Srinivas | Rajiv Gandhi Centre for Biotechnology (RGCB) |
| SKU: | 161594 |
|---|---|
| Product description: | MCF-7 scrambled RNA Control cell line |
| Gender: | Female |
| CRISPR: | Yes |
| Conditional: | Yes |
| Conditional description: | conditional BRCA1 Hypermethylated cell line which gets reverted in the presence of tetracycline/doxycycline |
| Production details: | Cells with wildtype unmethylated BRCA1 background were infected with lentiviruses that encode for deadCas9- DNMT3A fusion protein and synthetic guide RNAs under the control of a tet-off promoter. After successful infection, the cells were then established following FACS sorting. |
| Additional notes: | 1?g/ml of doxycycline for 12 hours can stop the eGFP signal from pCL-CTIG and BRCA1 hypermethylation will be reverted. |
| Receptors of note: | Expression of BRCA1 only in the presence of doxycycline |
| Parental cell line: | MCF7 |
| Model description: | BRCA1 hypermethylated cells developed using CRISPR-Cas9 technology |
| Disease: | Cancer |
| Cat. #: | 161594 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Eipgenetic modification |
| Morphology: | Epithelial slightly elongated morphology |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast carcinoma |
| Primary citation: | https://doi.org/10.1101/2022.04.30.490082 |
| Initial handling information: | Store in Liquid Nitrogen. Thaw in 10% DMEM with 1% Penicillin-Streptomycin |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM medium supplemented with 10% FBS and 1% Penicillin-Streptomycin |
| Subculture routine: | Split the cells into 1:3 ratio when the cells reach 80% Confluency |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Storage medium: | 90% FBS + 1% Penicillin-Streptomycin + 10% sterile DMSO |
| Recommended controls: | MCF-7 sgRNA Control cell line, MCF-7 sgRNA1 controls Control cell line , MCF-7 sgRNA2 Control cell line |
| Biosafety level: | 2 |
| References: |
https://doi.org/10.1101/2022.04.30.490082 |
|---|
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