K38 Keratinocyte Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: K38 Keratinocyte Cell Line
• Research fields: Drug development
• Organism: Mouse
• Disease: Dermatology
• Growth properties: Adherent
• Model: Primary line
• Description: The K38 Keratinocyte Cell Line has been established from the skin of neonatal WT mice. The prospective commercial application of the line is in the generation of in vitro epidermal 'skin equivalents' to be used as screening tools to look at the activity of NCE's and/or NBE's of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments Murine keratinocyte stem cell line
• Production details: Keratinocytes were isolated from neonatal wild-type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
• Cellosaurus id: CVCL_W141

Handling

• Format: Frozen
• Growth medium: The cell line is grown in low calcium FAD medium (FAD: DMEM/HAM’s F12, 3.5:1.1, with 0.05 mM Ca2+) supplemented with 10% FBS / FCS [Chelex 100 (Biorad)-treated to remove Ca2+ ions], 0.18 mM adenine, 0.5 μg/mL hydrocortisone, 5 μg/mL insulin,10 -10 M cholera toxin, 10 ng/mL EGF, 2 mM L-Glutamine, 1 mM pyruvate, 100 U/ml penicillin and 100 µg/ml streptomycin.
• Temperature: 32°C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Wash monolayer with PBS (without Ca2+ and Mg2+), incubate with EDTA solution (0.02% EDTA) for 5 min at room temperature and then incubate for 8-12 min with 0.05% trypsin/0.02% EDTA at 37°C until the cells come off easily. Add complete FAD medium and resuspend the cells by pipetting up and down 3-5 times. After centrifugation for 5 min at 220 x g, resuspend the pellet in complete FAD medium, and seed the cells at 3-5 x 10^4 cells/cm2 onto collagen I-coated plastic culture dish. Cultures are split 1:2 or 1:3, at confluency/2-3 times per week. Please also see detailed protocol within the Product Datasheet in the Documentation section below.

References

• Vollmers et al. 2012. Stem Cell Rev. 8(2):402-13. PMID: 21892602
• Hoher et al. 2012. Stem Cell Rev. 8(2):426-34. PMID: 21874280
• Reichelt et al. 2010. Methods Mol Biol. 585:59-69. PMID: 19907996

Documentation

• Product Datasheet