Immortalised Human Keratinocyte Cell Line

Immortalised human keratinocytes are widely used for studies of skin biology and differentiation

Contributors

Inventor
Pranab K Das
Cat. #: 154113
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Human
Tissue: Kidney
Model: Immortalised Line
Growth properties: Cells were routinely passaged 1:4 at confluency
Product description: Immortalised human keratinocytes are widely used for studies of skin biology and differentiation
Production details: Keratinocyte cultures were establised from neonatal foreskin using standard methods. Keratinocytes were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
Parental cell line: Human foreskin
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Dulbeccoâ?‚€?‚™s Modified Eagle Medium [High Glucose, 2mM L-glutamine] supplemented with 10% Fetal Calf Serum

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