Immorta-MAIT T cell clone MG-A4 cell line

Immorta-MAIT T cell clone MG-A4 cell line: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in […]

Contributors

Inventor Institute
Marielle Gold ; Erin Meerseier ; Irina Kurtz ; David Lewinsohn Oregon Health & Science University (OHSU)
Cat. #: 159691
Unit size: 1×10^6 cells / vial
Research Fields: Immunology
Organism: Human
Model: Immortalised Line
Growth properties: TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This clone expresses CD4, CD8, CD161, and CD27 and can be classified as a double-positive thymocyte. This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1– A549 cell line.
Product description: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
Conditional: No
Production details: Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.
disease: Mycobacterium tuberculosis
Format: Frozen
Shipping conditions: Dry ice
Growth medium: These cell lines are not suitable for large-scale expansion following standard cell culture protocols. Following the recommended guidelines the cells should double after a week, after which cells are stable and useable for weeks. <br> <br> Upon receipt spin cells and resuspend them in 15ml of fresh media and add to T12.5 flask. Recommended media is RPMI supplemented with 10% FBS, L-Glut, gentamicin and 0.5ng/mL IL2. Incubate at 37 °C. Aspirate half the media and replace with fresh media every 3-4 weeks.

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