IDHmut glioma stem-like NCH551b cell line

Glioma stem-like cell line NCH551b expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas.

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
Cat. #: 162301
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Grown as free-floating neurospheres under serum-free culture conditions.
Growth properties: Cells grow as free-floating neurospheres under serum-free conditions in non-adherent culture vessels. Cultures are maintained at 37°C in a humidified incubator with 5% CO2.
Primary citation: Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
Product description: NCH551b is a patient-derived glioma stem?like cell line with an IDH mutation, derived from the second relapse of an astrocytoma that progressed from WHO grade II to WHO grade IV. Tumour tissue was obtained from a 40 year old male patient-deriveduring surgical resection. The cell line is maintained under serum-free conditions and grows as free floating neurospheres.
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Storage conditions: FCS-free freezing medium (Synth-a-freeze medium)
Growth medium: DMEM/F-12–based serum-free medium supplemented with BIT, GlutaMAX, EGF, and FGF-2.
Subculture routine: Neurospheres are passaged at high cell density by gentle mechanical dissociation. Accutase may be used if required to obtain single-cell suspensions. Cells are reseeded in fresh serum-free medium.
Temperature: 37°C
References:

Trong et al. 2023. Cells. 12(8).

Cano-Galiano et al. 2021. Neurooncol Advances. 3(1). PMID: 34250481.

Ricklefs et al. 2020. Int J Mol Sci. 21(6).

Trong et al. 2020. Cells. 9(6).

Biedermann et al. 2019. Cancers. 11(12).

Trong et al. 2018. Int J Mol Sci. 19(10):2903. PMID: 30257451.

Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.

Bunse et al. 2018. Nat Med. 24(8):1192-1203. PMID: 29988124.

Pusch et al. 2017. Acta Neuropathol. 133(4):629-644. PMID: 28124097.

Kohanbash et al. 2017. J Clin Invest. 127(4):1425-1437. PMID: 28319047.

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