HEK293-A GFP-WIPI2 Cell Line

The HEK293-A GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151716
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Drug development;Metabolism
Organism: Human
Tissue: Embryo
Model: Transgenic
Growth properties: Autophagy
Alternate name: HEK293-A GFP-WIPI2
Product description: The HEK293-A GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI2 in background of HEK293-A cell line
Production details: HEK293-A cells were transfected with GFP-WIPI2 then selcted with G418, and stable, amino acid responsive clones were selected.
Additional notes: HEK293 cells were obtained under licence from AdVec
Cellosaurus ID: CVCL_5A21
Parental cell line: HEK293-A
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM with 10% FCS
Recommended controls: HEK293-A parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.

WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.

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