#151716

HEK293-A GFP-WIPI2 Cell Line

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Cat. #151716

HEK293-A GFP-WIPI2 Cell Line

Cat. #: 151716

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Embryo

Disease: Cancer

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sharon Tooze

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HEK293-A GFP-WIPI2 Cell Line
  • Research fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Drug development;Metabolism
  • Tool sub type: Continuous
  • Parental cell: HEK293-A
  • Organism: Human
  • Tissue: Embryo
  • Disease: Cancer
  • Growth properties: Autophagy
  • Model: Transgenic
  • Description: The HEK293-A GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI2 in background of HEK293-A cell line
  • Production details: HEK293-A cells were transfected with GFP-WIPI2 then selcted with G418, and stable, amino acid responsive clones were selected.
  • Biosafety level: 1
  • Additional notes: HEK293 cells were obtained under licence from AdVec
  • Recommended controls: HEK293-A parental line
  • Cellosaurus id: CVCL_5A21

Target Details

  • Target: Human WIPI2, GFP

Handling

  • Format: Frozen
  • Growth medium: DMEM with 10% FCS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: HEK293-A GFP-WIPI1 Cell Line

References

  • Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.
  • WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.