HEK293-A GFP-WIPI1 Cell Line

The HEK293-A GFP-WIPI1 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI1 in background of HEK293-A cell line

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151717
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Drug development;Metabolism
Organism: Human
Tissue: Embryo
Model: Transgenic
Growth properties: Autophagy
Product description: The HEK293-A GFP-WIPI1 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI1 in background of HEK293-A cell line
Production details: HEK293-A cells were transfected with GFP-WIPI1 then selcted with G418, and stable, amino acid responsive clones were selected.
Additional notes: HEK293-A cells were obtained under licence from AdVec
Cellosaurus ID: CVCL_5F67
Parental cell line: HEK293-A
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM with 10% FCS
Recommended controls: HEK293-A parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.

WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.

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