HCT 116 EphA2 KO Tet-inducible Cell Line

EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the…

Contributors

Inventor Institute
Sandra Van Schaeybroeck Queen's University Belfast
Cat. #: 153207
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Colon
Model: Knock-Out
Growth properties: Invasion, migration
Cancer Types In Detail: Colorectal
Product description: EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
Conditional: Yes
Conditional description: Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline
Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
Cellosaurus ID: CVCL_HG05
Parental cell line: HCT 116
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
Recommended controls: HCT 116 parental line
References:

Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.

EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.

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