#153207

HCT 116 EphA2 KO Tet-inducible Cell Line

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Cat. #153207

HCT 116 EphA2 KO Tet-inducible Cell Line

Cat. #: 153207

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Knock-Out

£575.00

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Contributor

Inventor: Sandra Van Schaeybroeck

Institute: Queen's University Belfast

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HCT 116 EphA2 KO Tet-inducible Cell Line
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Colorectal
  • Research fields: Cancer;Cell biology;Drug development
  • Tool sub type: Continuous
  • Parental cell: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Knock-Out
  • Conditional: Yes
  • Conditional description: Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline
  • Description: EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
  • Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
  • Recommended controls: HCT 116 parental line
  • Cellosaurus id: CVCL_HG05

Target Details

  • Target: EphA2

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
  • EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.