The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
| Inventor | Institute |
|---|---|
| Sandra Van Schaeybroeck | Queen's University Belfast |
| SKU: | 153207 |
|---|---|
| Product description: | EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion. |
| Conditional: | Yes |
| Conditional description: | Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline |
| Production details: | HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÄÂÄ?g/mL puromycin and induced with 2ÄÂÄ?g/ml doxycycline. |
| Cellosaurus ID: | CVCL_HG05 |
| Parental cell line: | HCT 116 |
| Disease: | Cancer |
| Cat. #: | 153207 |
|---|---|
| Provenance and Ownership: | The HCT 116 EphA2 KO Tet-inducible Cell Line is owned by Cancer Research UK. CancerTools supplies the HCT 116 EphA2 KO Tet-inducible Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HCT 116 EphA2 KO Tet-inducible Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Colorectal cancer |
| Research Fields: | Cancer; Cell biology; Drug development |
| Organism: | Human |
| Tissue: | Colon |
| Model: | Knock-Out |
| Growth properties: | Invasion, migration |
| Cancer Types In Detail: | Colorectal |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | McCoy’s 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin |
| Recommended controls: | HCT 116 parental line |
| References: |
Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684. EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer. |
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