HCT 116 AXL KO Tet-inducible Cell Line

The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.

Contributors

Inventor Institute
Sandra Van Schaeybroeck Queen's University Belfast
Cat. #: 153206
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Colon
Model: Knock-Out
Growth properties: Invasion, migration
Cancer Types In Detail: Colorectal
Product description: Receptor tyrosine kinase (RTK) screens identifed AXL as a protein which underpins the migratory/invasive phenotype in colorectal cancer (CRC) (Dunne et al., 2014). AXL was shown to be a poor prognostic marker and an important mediator of cell migration/invasiveness. The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.
Conditional: Yes
Conditional description: Conditional knockdown of endogenous AXL expression upon treatment with Tetracycline
Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against AXL. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
Cellosaurus ID: CVCL_HG04
Parental cell line: HCT 116
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
Recommended controls: HCT 116 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.

AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.

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