#153206

HCT 116 AXL KO Tet-inducible Cell Line

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Cat. #153206

HCT 116 AXL KO Tet-inducible Cell Line

Cat. #: 153206

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sandra Van Schaeybroeck

Institute: Queen's University Belfast

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HCT 116 AXL KO Tet-inducible Cell Line
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Colorectal
  • Research fields: Cancer;Cell biology;Drug development
  • Tool sub type: Continuous
  • Parental cell: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Knock-Out
  • Conditional: Yes
  • Conditional description: Conditional knockdown of endogenous AXL expression upon treatment with Tetracycline
  • Description: Receptor tyrosine kinase (RTK) screens identifed AXL as a protein which underpins the migratory/invasive phenotype in colorectal cancer (CRC) (Dunne et al., 2014). AXL was shown to be a poor prognostic marker and an important mediator of cell migration/invasiveness. The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.
  • Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against AXL. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
  • Biosafety level: 1
  • Recommended controls: HCT 116 parental line
  • Cellosaurus id: CVCL_HG04

Target Details

  • Target: AXL

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
  • AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.