Cat. #153425
H413 Cell Line
Cat. #: 153425
Unit size: 1x10^6 cells / vial
Tissue: Buccal mucosa
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stephen Prime
Institute: University of Bristol
Tool Details
*FOR RESEARCH USE ONLY
- Name: H413 Cell Line
- Tissue: Buccal mucosa
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a 53 year-old female patient. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 68 exon 4, G to T; wild type K-, N- and Ha-ras. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth
- Cellosaurus id: CVCL_2465
Target Details
- Target: Human oral squamous cell carcinoma, buccal mucosa
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?°C. Suggested seeding density 5 x 1000 cells/cm? . Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (...
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
- Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
- Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
- TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
- Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
- Epidermal growth f...