Glioblastoma WHO IV spheroid cell line, NCH601

Glioblastoma WHO IV spheroid cell line, NCH601

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
Cat. #: 162325
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Grown as free-floating neurospheres under serum-free culture conditions.
Growth properties: Cells grow as free-floating neurospheres under serum-free conditions in non-adherent culture vessels. Cultures are maintained at 37°C in a humidified incubator with 5% CO2.
Product description: NCH601 is a patient-derived IDH wildtype glioblastoma neurosphere cell line established from a primary WHO grade IV tumour obtained from a 76 year old male patient. The tumour has been classified as proneural subtype (PN, RTKII) and was reported as MGMT promoter negative. Cells grow as free floating neurospheres under serum-free conditions.
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium: DMEM/F-12–based serum-free medium supplemented with BIT, GlutaMAX, EGF, and FGF-2.
Subculture routine: Neurospheres are passaged at high cell density by gentle mechanical dissociation. Accutase may be used if required to obtain single-cell suspensions. Cells are reseeded in fresh serum-free medium.
Temperature: 37°C
References:

Remy et al. 2022. Cancers. 14(2):339. PMID: 35053502.

Cano-Galiano et al. 2021. Neurooncol Advances. 3(1). PMID: 34250481.

Schuster et al. 2020. Nat Commun. 11(1):6366. PMID: 33311477.

Golebiewska et al. 2020. Acta Neuropathol. 140(6):919-949. PMID: 33009951.

Fritah et al. 2020. Cancers. 12(9):2583. PMID: 32927769.

Biedermann et al. 2019. Cancers. 11(12):2028. PMID: 31888244.

Rusu et al. 2019. Cell Stem Cell. 25(2):241-257. PMID: 31303549.

Trong et al., 2018. Int J Mol Sci. 19(10):2903. PMID: 30257451.

Schulze et al. 2018. BMC Cancer. 18(1):524. PMID: 29724193.

Hoja et al. 2016. Oncotarget. 7(39):62989-63002. PMID: 27556305.

Chiblak et al. 2016. Int J Radiat Oncol Biol Phys. 95(1):112-119. PMID: 26254681.

Bougnaud et al. 2016. Oncotarget. 7(22):31955-31971. PMID: 27049916.

Dokic et al. 2012. Glia. 60(11):1785-1800. PMID: 22951908.

Campos et al. 2014. J Pathol. 234(1):23-33. PMID: 24756862.

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