Glioblastoma WHO IV NCH474 cell line

Glioblastoma WHO IV cell line, NCH474

Contributors

Institute
Heidelberg University
Cat. #: 162319
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37°C in a humidified incubator with 5% CO?. Medium is replaced twice per week.
Primary citation: Campos et al. 2015. Glia. 63(10):1850-1859. PMID: 25944104.
Product description: NCH474 is a patient-derived IDH?wildtype glioblastoma cell line established from a primary WHO grade IV tumour resected from a 65?year?old female patient. Cells grow as adherent monolayer cultures under standard conditions.
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Campos et al. 2015. Glia. 63(10):1850-1859. PMID: 25944104.

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