Glioblastoma WHO IV NCH125 cell line

Glioblastoma WHO IV cell line, NCH125

Contributors

Institute
Heidelberg University
Cat. #: 162309
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Adherent
Growth properties: Cells grow as adherent monolayer cultures under standard culture conditions at 37?°C in a humidified incubator with 5% CO?. Medium is replaced twice per week.
Primary citation: Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.
Product description: NCH125 is a patient-derived IDH wildtype glioblastoma cell line established from a primary WHO grade IV tumour resected from a 43 year old male patient. Cells grow as adherent monolayer cultures under standard conditions
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Growth medium: DMEM (high glucose) supplemented with 10% fetal calf serum (FCS), 1% GlutaMAX, and 1% penicillin/streptomycin.
Subculture routine: Cells are passaged at sub-confluent densities using 0.05% Trypsin-EDTA. After enzymatic detachment, trypsin is neutralised with serum-containing medium and cells are reseeded into fresh culture vessels. Passage number should be recorded.
Temperature: 37°C
References:

Fermi et al. 2022. Chemistry. 28(34):e202104535. PMID: 35293640.

Dokic et al. 2012. Glia. 60(11):1785-1800. PMID: 22951908.

Schule et al. 2012. Scientific World Journal. 2012:697313. PMID: 22919349.

Siegelin et al. 2008. Mol Cancer Ther. 7(11):3566-3574. PMID: 19001439.

Karcher et al. 2005. Cancer Cell Biol. 118(9):2182-2189. PMID: 16331629.

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