A murine MUC1/STn-positive breast cancer model used to study tumour-associated glycans, antibody responses, and preclinical immunotherapy. Exclusive to CancerTools.
| Inventor | Institute |
|---|---|
| Joyce Taylor-Papadimitriou ; Joy Burchell | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 151847 |
|---|---|
| Product description: | The E3 STn Cell Line is a mouse mammary carcinoma cell line E3 which expresses human MUC1 and Sialyl-Tn (STn) and can be used for vaccine and immunotherapy development. Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn is an O-linked disaccharide that can be carried on various glycoproteins including MUC1 which is highly expressed in breast cancer. Both STn and MUC1 are considered promising targets for immunotherapy for the treatment of breast cancer. |
| Conditional: | Yes |
| Production details: | E3 cell line stably transfected with murine ST6GalNAc I leading to expression of the MUC1 STn epitope. The E3 cell lines were developed from the 410.4 cell line by transfection of the hygromycin resistance gene with the MUC1 gene. Calcium phosphate transfection of E3 cells was performed using the pbabe-neo gene with or without the cDNA encoding murine B7.1. Stable transfection of murine ST6GalNAc I was performed to obtain the cell line E3-STn. |
| Additional notes: | E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn |
| Cellosaurus ID: | CVCL_W346 |
| Parental cell line: | E3 |
| Disease: | Cancer |
| Cat. #: | 151847 |
|---|---|
| Provenance and Ownership: | The E3 STn Cell Line is owned by Cancer Research UK. CancerTools supplies the E3 STn Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing E3 STn Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Organism: | Mouse |
| Tissue: | Breast |
| Model: | Knock-In |
| Cancer Types In Detail: | Breast cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Dulbecco’s E4 medium containing 600 µg/ml G418, 200 µg/ml hygromycin and 10% fetal calf serum (FCS) |
| Recommended controls: | E3 parental line |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292. |
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